microRNA precursor processing

microRNA precursor processing

Details

Our group identified and determined the cellular location of one of the proteins, HYL1, involved in miRNA precursor processing in Arabidopsis.  We later established the first in vitro plant microRNA precursor processing system and identified structural features of RNA precursors critical for correct processing.  We are currently engaged in characterizing the protein-protein and protein-RNA interactions involved in miRNA precursor processing using highly purified proteins.  These experiments are done in collaboration with Dr. Samir Hamdan.

Publications

  • Liu, C., Axtell, M. J. and Fedoroff, N. V. (2012). The helicase and RNaseIIIa domains of Arabidopsis DCL1 modulate catalytic parameters during microRNA biogenesis. Plant Physiol 159: 748-58. PDF
  • Song, L., Axtell, M. J. and Fedoroff, N. V. (2010) Secondary structural determinants of MIRNA precursor processing in Arabidopsis. Cur. Biol. 20 :37-41.  PDF
  • Dong, Z., Han, M.-H., and Fedoroff, N. V. (2008). The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1.  Proc. Natl. Acad. Sci. USA 105: 9970-5. PDF
  • Song, L., Han, M.-H., Lesicka, J., and N. Fedoroff (2007).  Arabidopsis primary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body. Proc. Natl. Acad. Sci. USA 104: 5437-5442. PDF
  • Han, M.-H., Goud, S., Song, L., and Fedoroff, N. (2004) The Arabidopsis dsRNA-binding protein HYL1 plays a role in microRNA-mediated gene regulation. Proc. Natl. Acad. Sci. USA, 101: 1093-1098. PDF
  • Lu, C. and N. Fedoroff (2000). A mutation in the Arabidopsis HYL1 gene encoding a dsRNA binding protein affects responses to abscisic acid, auxin, and cytokinin. Plant Cell 12:2351-2366.  [Cited in Editor’s Choice, Science, 291: 557 (2001)]. PDF